AS with waiting time 5 seconds and B without waiting time. Pipettes are adjusted to deliver of its indicated capacity; it means the volume measured by the pipette is the volume that runs out from the pipette.
The rest of the liquid that remains on the pipette walls and in the jet is not included to the measured volume. These vessels calibrated to contain. There are two basic types of pipettes: The volumetric pipettes are used for the dosing of nominal volume given on the pipette label. The graduated pipettes can dose any volume within the nominal pipette volume. Volumetric pipettes with nominal volume up to ml are made from soda-lime glass. Volumetric pipettes with the volume up to ml are produced from borosilicate glass.
The pipettesare enlarged in the center part pipettes with volume 1 and 2 ml are also produced without enlargement in the center part and they have one graduation line on the upper part of the tube above the enlargement; in the case of two-sign pipettes could have one more graduation line on the tube part below the enlargement.
The height of liquid level is adjusted after the filling the tube in such a way that meniscus will touch upper edge of the graduation line, see Figure No. Join of bottom edge of meniscus and eye must be perpendicular to the longitudinal axis of the pipette during the adjustment the meniscus, see Figure No.
The liquid is then discharged from the pipette completely up to the jet of pipette, in case of two sign pipettes up to the second sign. In case of pipettes with B class of accuracy the dosing of measured volume can be stopped after the releasing of the liquid from the pipette. Graduated pipettes of the volume up to 25 ml are made from soda-lime glass, graduated pipettes of the volume 50 and ml are produced from borosilicate glass.
The scale is printed by silkscreen printing on the tube and each tenth mark is numerated. The scale is divided in milliliters, pertinently in fractions of milliliters.
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